An Enzyme Controlled Reaction-Catalase Essay Example | Topics and Well Written Essays - 1000 words

An Enzyme Controlled Reaction-Catalase - Essay spokespersonl. Compute the value of O2 production (cm3/s). m. Plot a graph of concentration of heat content peroxide against rate of oxygen production. 5.0 Safety Measures Hydrogen peroxide in high concentrations (18-28 vol.) is irritating to the eye and the skin. The contaminants can fix decomposition leading to explosion after a while. Pressure can build up if reply containers are blocked. 6.0 Results This experiment has 1 breakaway variable, 1 dependent variable and 1 control variable. The independent variable is the various concentrations of hydrogen peroxide, while the dependent variable is the rate of reaction in call of oxygen production. The control variable is the temperature of the room and standard pressure. put off 1 shows the average results of the oxygen produced in different concentrations of hydrogen peroxide. Percentage of Hydrogen peroxide Average volume of oxygen collected (cm3) all over time (seconds) 5 10 15 20 25 30 35 40 45 50 55 60 deoxycytidine monophosphate 48.3 68.7 78.3 82.0 85.0 86.7 87.0 87.3 87.7 88.3 88.3 88.3 90 37.3 55.0 63.7 68.3 71.0 72.3 73.0 73.3 73.3 73.3 73.3 73.3 80 32.3 47.3 54.0 59.0 61.3 63.0 63.3 63.7 63.7 63.7 63.7 63.7 70 26.3 40.0 45.7 48.3 49.0 49.3 49.3 49.3 49.3 49.3 49.3 49.3 60 20.7 31.7 36.7 40.0 42.7 44.0 44.7 44.7 44.7 44.7 44.7 44.7 50 16.3 27.0 31.0 33.3 35.3 36.3 37.0 37.0 37.0 37.0 37.0 37.0 Table 1 Average volume of oxygen collected Figure 2 reaction at 100% concentration of hydrogen peroxide Figure 3 reaction at 80% concentration of hydrogen peroxide Figure 3 reaction at 50% concentration of hydrogen peroxide 7.0 intervention To ensure that the process of measuring was completed, I ensured the room temperature remained constant and the equipment remained in the very(prenominal) conditions for all the tests. In some tests, the level of concentration... To ensure that the process of measuring was accurate, I ensured the room temperature remain ed constant and the equipment remained in the same conditions for all the tests. In some tests, the level of concentration of hydrogen peroxide was not very accurate due to some technical hitches, but the level of inaccuracy was not substantial enough to affect the findings and coda of the experiment. From the results, it appears that less oxygen was produced within the first 5 seconds because the concentration was low. This is because, at higher concentrations of hydrogen peroxide, in that location are more molecules of hydrogen peroxide leading to more collisions that stimulates more enzyme-substrate to be formed as the concentration decreased, this collision decelerated as evidenced by the graphs (Chelikani, Fita & Loewen, 2004). These results are exactly as it was expected. Catalase functions as catalyses in most living organisms, by decomposing hydrogen peroxide into oxygen and water, as shown in the equation below.2H2O2 + Catalase 2H2O + O2Catalase significantly trims down the activation power required for the reaction. In absence of catalase, the breakdown would take a eight-day time to occur, and would not be able to support life. Since Hydrogen peroxide is a very hefty by-product of metabolism, if not decomposed very fast it can cause damage to body cells.